Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood. J Vis Exp 2022 Jan 06(179)
Date
01/25/2022Pubmed ID
35068481Pubmed Central ID
PMC9645770DOI
10.3791/63285Scopus ID
2-s2.0-85123744701 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
The endothelium is a dynamic integrated structure that plays an important role in many physiological functions such as angiogenesis, hemostasis, inflammation, and homeostasis. The endothelium also plays an important role in pathophysiologies such as atherosclerosis, hypertension, and diabetes. Endothelial cells form the inner lining of blood and lymphatic vessels and display heterogeneity in structure and function. Various groups have evaluated the functionality of endothelial cells derived from human peripheral blood with a focus on endothelial progenitor cells derived from hematopoietic stem cells or mature blood outgrowth endothelial cells (or endothelial colony-forming cells). These cells provide an autologous resource for therapeutics and disease modeling. Xenogeneic cells may provide an alternative source of therapeutics due to their availability and homogeneity achieved by using genetically similar animals raised in similar conditions. Hence, a robust protocol for the isolation and expansion of highly proliferative blood outgrowth endothelial cells from porcine peripheral blood has been presented. These cells can be used for numerous applications such as cardiovascular tissue engineering, cell therapy, disease modeling, drug screening, studying endothelial cell biology, and in vitro co-cultures to investigate inflammatory and coagulation responses in xenotransplantation.
Author List
Shradhanjali A, Uthamaraj S, Dragomir-Daescu D, Gulati R, Sandhu GS, Tefft BJAuthor
Brandon J. Tefft PhD Associate Professor in the Biomedical Engineering department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAtherosclerosis
Blood Coagulation
Cell- and Tissue-Based Therapy
Endothelial Progenitor Cells
Swine
Tissue Engineering
