Differentiation of murine embryonic stem cells induces progesterone receptor gene expression. Exp Cell Res 2005 Dec 10;311(2):251-64
Date
10/15/2005Pubmed ID
16223481Pubmed Central ID
PMC1350973DOI
10.1016/j.yexcr.2005.09.005Scopus ID
2-s2.0-27744549327 (requires institutional sign-in at Scopus site) 29 CitationsAbstract
The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERalpha, ERbeta) are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen-dependent manner by endogenous ERs or by transiently overexpressed ERalpha. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation.
Author List
Sauter CN, McDermid RL, Weinberg AL, Greco TL, Xu X, Murdoch FE, Fritsch MKAuthor
Carley N. Sauter MD Associate Professor in the Physical Medicine and Rehabilitation department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBase Sequence
Cell Differentiation
Cells, Cultured
Embryo, Mammalian
Gene Expression Regulation, Developmental
Homeodomain Proteins
Interleukin-6
Leukemia Inhibitory Factor
Mice
Molecular Sequence Data
Receptors, Progesterone
Stem Cells
Transcriptional Activation