Rapid identification of orexin receptor binding ligands using cell-based screening accelerated with magnetic beads. Mol Biosyst 2010 Jan;6(1):102-7
Date
12/22/2009Pubmed ID
20024071Pubmed Central ID
PMC3379552DOI
10.1039/b915611aScopus ID
2-s2.0-72949103678 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
We report here a simple and rapid method by which to screen one bead one compound libraries for highly specific ligands to cell surface proteins such as G protein-coupled receptors. This protocol, which harvests "hits" in a cell-based binding screen magnetically, eliminates the most tedious aspects of previously published bead screening techniques and allows millions of different compounds to be screened rapidly and cheaply. The method is demonstrated using the orexin receptor 1, which resulted in the isolation of moderate potency antagonists.
Author List
Qi X, Astle J, Kodadek TMESH terms used to index this publication - Major topics in bold
AnimalsCHO Cells
Cricetinae
Cricetulus
Ligands
Magnetics
Microspheres
Models, Biological
Molecular Structure
Orexin Receptors
Protein Binding
Receptors, G-Protein-Coupled
Receptors, Neuropeptide