[Uridine transport and phosphorylation in 3T3 and CHO cells with induced cell proliferation]. Tsitologiia 1981 May;23(5):523-30
Date
05/01/1981Pubmed ID
6167039Scopus ID
2-s2.0-0019569799 (requires institutional sign-in at Scopus site)Abstract
In the serum-deficient medium, the cultured Swiss 3T3 and CHO-K1 cells transit to the resting state. The rates of uridine phosphorylation and RNA synthesis in these cells are lowered. After the addition of fresh medium containing 10% serum, cell proliferation is induced. At the early stage of cell entrance into the cell cycle uridine transport through the cell plasma membrane remains unchanged in both cultures. During the 1st hour after serum addition the rate of uridine phosphorylation increases in 3T3 cells to remain practically unchanged in CHO-K1 cells. At this time, RNA synthesis in cells increases almost twofold in both cultures. A correlation has been revealed between the initial level of uridine phosphorylation in 3T3 cells and the percentage of its maximal elevation after serum addition. No such a correlation was observed for CHO-K1 cells. The rate of uridine phosphorylation in arrested CHO-K1 cells is higher than that in 3T3 cells. It has been included that the initial increase of uridine phosphorylation during serum stimulation may be not obligatory for all cell types, but depends on the level of uridine kinase activity before serum addition to the cells.
Author List
Sorokin AB, Sorkin AD, Nikol'skiĭ NNAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBiological Transport
Cell Division
Cells, Cultured
Cricetinae
Culture Media
DNA
Immune Sera
Mice
Oxidative Phosphorylation
RNA
Uridine
