Faculty Collaboration Database

Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells. Hepatology 2010 Jan;51(1):297-305

Date

12/10/2009

Pubmed ID

19998274

Pubmed Central ID

PMC2946078

DOI

10.1002/hep.23354

Scopus ID

2-s2.0-73449100783 (requires institutional sign-in at Scopus site)   1011 Citations

Abstract

UNLABELLED: There exists a worldwide shortage of donor livers available for orthotropic liver transplantation and hepatocyte transplantation therapies. In addition to their therapeutic potential, primary human hepatocytes facilitate the study of molecular and genetic aspects of human hepatic disease and development and provide a platform for drug toxicity screens and identification of novel pharmaceuticals with potential to treat a wide array of metabolic diseases. The demand for human hepatocytes, therefore, heavily outweighs their availability. As an alternative to using donor livers as a source of primary hepatocytes, we explored the possibility of generating patient-specific human hepatocytes from induced pluripotent stem (iPS) cells.

CONCLUSION: We demonstrate that mouse iPS cells retain full potential for fetal liver development and describe a procedure that facilitates the efficient generation of highly differentiated human hepatocyte-like cells from iPS cells that display key liver functions and can integrate into the hepatic parenchyma in vivo.

Author List

Si-Tayeb K, Noto FK, Nagaoka M, Li J, Battle MA, Duris C, North PE, Dalton S, Duncan SA

Author

Paula E. North MD, PhD Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Cell Differentiation
Hepatocytes
Humans
Induced Pluripotent Stem Cells
Mice