Faculty Collaboration Database

Usefulness of multiplex PCR in the diagnosis of genital tuberculosis in females with infertility. Eur J Clin Microbiol Infect Dis 2013 Mar;32(3):399-405

Date

10/12/2012

Pubmed ID

23052988

DOI

10.1007/s10096-012-1755-y

Scopus ID

2-s2.0-84874114273 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

The purpose of this investigation was to evaluate the usefulness of multiplex polymerase chain reaction (m-PCR) in detecting uterine tuberculosis in women with infertility. In a prospective study, endometrial curetting from 620 females with infertility were investigated using laparoscopy, hysteroscopy, histopathology, smear microscopy, mycobacterial culture in BACTEC MGIT™ 960, and in-house m-PCR. The mean age of the women was 29.75 ± 4.66 years. The majority (596) sought medical attention for infertility; of them, 455 (76.34 %) presented with primary and 141 (23.65 %) with secondary infertility. A total of 158 (25.48 %) women were diagnosed as having uterine tuberculosis by at least one of the diagnostic methods. Among them, laparoscopy was positive in 46 (29.11 %), hysteroscopy in 77 (48.73 %), histopathology in only 8 (5.06 %), smear for acid fast bacilli in 4 (2.53 %), and liquid culture in 24 (15.18 %) patients. The in-house m-PCR was positive in 135 (85.44 %) women. Of these, 129 (95.55 %) samples were positive for Mycobacterium tuberculosis, while 6 (4.44 %) were positive for non-tuberculous mycobacterial DNA. Of the 129 M. tuberculosis PCR-positive women, 112 received anti-tubercular treatment and 23 of these conceived and fell pregnant after the completion of treatment. For the diagnosis of uterine tuberculosis, m-PCR was found to be the most efficient diagnostic tool compared to the other methods.

Author List

Sankar MM, Kumar P, Munawwar A, Kumar M, Singh J, Singh A, Parashar D, Malhotra N, Duttagupta S, Singh S

Author

Deepak Parashar PhD Assistant Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Bacteriological Techniques
Female
Humans
Infertility
Molecular Diagnostic Techniques
Multiplex Polymerase Chain Reaction
Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Prospective Studies
Tuberculosis, Female Genital
Young Adult