Medical College of Wisconsin
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Platelet activation and erythrocyte lysis during brief exposure of blood to pathophysiological shear stress in vitro. Clin Hemorheol Microcirc 2017;67(2):159-172

Date

08/12/2017

Pubmed ID

28800323

DOI

10.3233/CH-170256

Scopus ID

2-s2.0-85034654980 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

BACKGROUND: Interaction of von Willebrand factor (VWF) with circulating platelets is the trigger for thrombosis in a region of arterial stenosis. These events are typically studied in vitro under conditions where platelets adhere to a VWF-coated surface. Our approach assesses platelet responses in the absence of adhesion.

OBJECTIVE: To characterize extent of platelet activation and erythrocyte lysis in an artificial stenosis model.

METHODS: Whole blood is perfused through a length of polyetheretherketone tubing that includes an artificial stenosis, comprising narrow-bore (89-381 μm) tubing. Secretion of [14C] serotonin and hemoglobin release was measured to evaluate platelet activation and hemolysis respectively at various perfusion rates and different stenosis dimensions.

RESULTS: Platelet activation and erythrocyte lysis increased progressively with increasing perfusion rate and decreasing stenosis diameter; the length of the stenosis had negligible influence. Modest platelet activation (5-10% secretion of [14C] serotonin) occurred without significant erythrocyte lysis under a limited range of perfusion conditions (4-6 mL/min flow through a 127 μm stenosis).

CONCLUSIONS: Our experimental approach mimics conditions in severe arterial stenosis or a mechanical heart valve. It could be a valuable aid in the development of novel drugs to treat arterial thrombosis and in the design of heart valves.

Author List

Majumdar S, Patil CN, Ladner-Threadgill T, Randolph E, Burgreen GW, Kermode JC

Author

Chetan N. Patil Research Scientist I in the Obstetrics and Gynecology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Blood Chemical Analysis
Blood Platelets
Erythrocytes
Humans
Platelet Activation
Platelet Adhesiveness
Stress, Mechanical