[Proliferation and DNA synthesis in a CHO-K1 cell culture with a varying serum concentration in the medium]. Tsitologiia 1981 Apr;23(4):468-72
Date
04/01/1981Pubmed ID
7256849Scopus ID
2-s2.0-0019555691 (requires institutional sign-in at Scopus site)Abstract
The possibility of using CHO-K1 cells for investigation of mechanisms involved in stimulation of cell proliferation by serum has been studied. The decrease of serum content in the cultural medium down to 0.5% causes the arrest of cell growth and the lowering of DNA synthesis. The addition of fresh 10% serum stimulates DNA synthesis 12 hours later with the following renewal of cell proliferation. G1-period in cells synchronized by vinblastine is 6 hours. Thus, the removal of some serum factors form cultural medium causes a reversible arresting of CHO-K1 cell proliferation.
Author List
Sorokin ABAuthor
Andrey Sorokin PhD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCattle
Cell Division
Cell Line
Cells, Cultured
Cricetinae
Culture Media
DNA
Dose-Response Relationship, Drug
Immune Sera
Stimulation, Chemical
Vinblastine
