Faculty Collaboration Database

Lipid fluidity modulates platelet aggregation and agglutination in vitro. Life Sci 1993;53(13):1053-60

Date

01/01/1993

Pubmed ID

8366768

DOI

10.1016/0024-3205(93)90258-5

Scopus ID

2-s2.0-0027220983 (requires institutional sign-in at Scopus site)   23 Citations

Abstract

To determine the effect of altered membrane fluidity on platelet aggregation/agglutination, fresh, washed human platelets were treated with A2C, a cyclopropyl fatty acid ester which is known to enhance mobility of intrinsic membrane bilayer constituents and increase membrane fluidity. Fluorescence polarization studies demonstrated A2C incubation time- and concentration-dependent increases in platelet membrane fluidity (decreased fluorescence anisotropy). Preincubation with A2C was associated with diminished collagen, thrombin and ristocetin-induced platelet aggregation/agglutination. Aggregation/agglutination was diminished by 93 +/- 5% for collagen (0.2 mg/ml), 53 +/- 3% for thrombin (1.0 U/ml) and 85 +/- 9% for ristocetin (1.1 mg/ml). These data suggest that membrane fluidity is involved in the regulation of platelet function.

Author List

Vlasic N, Medow MS, Schwarz SM, Pritchard KA Jr, Stemerman MB

Author

Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Agglutination
Animals
Cattle
Fluorescence Polarization
Humans
In Vitro Techniques
Membrane Fluidity
Platelet Aggregation
Stearates
Time Factors