Faculty Collaboration Database

Histamine stimulation of prostaglandin and HETE synthesis in human endothelial cells. Am J Physiol 1988 Aug;255(2 Pt 1):C214-25

Date

08/01/1988

Pubmed ID

3407766

DOI

10.1152/ajpcell.1988.255.2.C214

Scopus ID

2-s2.0-0023814623 (requires institutional sign-in at Scopus site)   48 Citations

Abstract

Endothelial cells (EC) cultured from human umbilical artery (UA) and vein (UV) metabolized [14C]arachidonic acid to prostaglandins (PGs), monohydroxyeicosatetraenoic acids (HETEs), and epoxyeicosatrienoic acids (EETs). Major radioactive products were identified as 6-keto-PGF1 alpha, PGE2, PGF2 alpha, 12-hydroxy heptadecatrienoic acid, 15-HETE, and 11-HETE. In addition, extracts from UV ECs contained 12-HETE, 5-HETE, 14,15-EET, and 5,6-EET as minor products, whereas extracts from UA ECs contained only 12-HETE as a minor product. UA ECs also produced metabolites comigrating with 14,15-EET, 11,12-EET, 8,9-EET, and 5,6-EET. Histamine increased the release of [14C]PGs and [14C]HETEs from [14C]arachidonic acid-labeled ECs. Indomethacin, aspirin, and nordihydroguauretic acid completely inhibited synthesis of both [14C]PGs and [14C]HETEs from exogenous [14C]arachidonic acid in these cells. Microsomes metabolized [14C]arachidonic acid to the same [14C]PGs and [14C]HETEs as intact cells. Pretreatment of microsomes with indomethacin completely inhibited formation of these products. These data indicate that UA ECs and UV ECs metabolize endogenous and exogenous arachidonic acid to both PGs and HETEs. Also 15-HETE and 11-HETE appear to be synthesized by a microsomal enzyme with the properties of cyclooxygenase.

Author List

Revtyak GE, Hughes MJ, Johnson AR, Campbell WB

Author

William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Arachidonic Acids
Carbon Radioisotopes
Cells, Cultured
Endothelium, Vascular
Female
Gas Chromatography-Mass Spectrometry
Histamine
Humans
Hydroxyeicosatetraenoic Acids
Kinetics
Microsomes
Pregnancy
Prostaglandins
Pyrilamine
Umbilical Arteries
Umbilical Veins