Faculty Collaboration Database

Suz12 binds to silenced regions of the genome in a cell-type-specific manner. Genome Res 2006 Jul;16(7):890-900

Date

06/06/2006

Pubmed ID

16751344

Pubmed Central ID

PMC1484456

DOI

10.1101/gr.5306606

Scopus ID

2-s2.0-33745604636 (requires institutional sign-in at Scopus site)   275 Citations

Abstract

Suz12 is a component of the Polycomb group complexes 2, 3, and 4 (PRC 2/3/4). These complexes are critical for proper embryonic development, but very few target genes have been identified in either mouse or human cells. Using a variety of ChIP-chip approaches, we have identified a large set of Suz12 target genes in five different human and mouse cell lines. Interestingly, we found that Suz12 target promoters are cell type specific, with transcription factors and homeobox proteins predominating in embryonal cells and glycoproteins and immunoglobulin-related proteins predominating in adult tumors. We have also characterized the localization of other components of the PRC complex with Suz12 and investigated the overall relationship between Suz12 binding and markers of active versus inactive chromatin, using both promoter arrays and custom tiling arrays. Surprisingly, we find that the PRC complexes can be localized to discrete binding sites or spread through large regions of the mouse and human genomes. Finally, we have shown that some Suz12 target genes are bound by OCT4 in embryonal cells and suggest that OCT4 maintains stem cell self-renewal, in part, by recruiting PRC complexes to certain genes that promote differentiation.

Author List

Squazzo SL, O'Geen H, Komashko VM, Krig SR, Jin VX, Jang SW, Margueron R, Reinberg D, Green R, Farnham PJ

Author

Victor X. Jin PhD Professor in the Data Science Institute department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Binding Sites
Carcinoma, Embryonal
Cell Differentiation
Chromatin
Chromatin Immunoprecipitation
Gene Expression Regulation, Developmental
Gene Silencing
Gene Targeting
Genetic Markers
Genome
Glycoproteins
Homeodomain Proteins
Humans
Immunoglobulins
Mice
Octamer Transcription Factor-3
Oligonucleotide Array Sequence Analysis
Polycomb Repressive Complex 2
Promoter Regions, Genetic
Repressor Proteins
Stem Cells
Transcription Factors