Permeability of nitric oxide through lipid bilayer membranes. Free Radic Res 1996 May;24(5):343-9
Date
05/01/1996Pubmed ID
8733938DOI
10.3109/10715769609088032Scopus ID
2-s2.0-0029973675 (requires institutional sign-in at Scopus site) 104 CitationsAbstract
Profiles of the local nitric oxide (.NO) diffusion-concentration product across the egg yolk phosphatidylcholine membrane in the absence and presence of 30 mol% cholesterol were obtained using line-broadening electron paramagnetic resonance (EPR) and lipid-soluble nitroxide spin labels. Membrane .NO permeability coefficients were calculated from these profiles. At 20 degrees C, values of 93 and 77 cm/s for membranes in the absence and presence of cholesterol were obtained, compared with 73 and 66 cm/s for water layers of the same thickness as the membranes. Fluid-phase membranes are not barriers to .NO transport. Cholesterol significantly increases .NO transport in the center of the lipid bilayer.
Author List
Subczynski WK, Lomnicka M, Hyde JSAuthor
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Biological TransportDiffusion
Electron Spin Resonance Spectroscopy
Lipid Bilayers
Molecular Probes
Nitric Oxide
Permeability
Phosphatidylcholines