Medical College of Wisconsin
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Permeability of nitric oxide through lipid bilayer membranes. Free Radic Res 1996 May;24(5):343-9

Date

05/01/1996

Pubmed ID

8733938

DOI

10.3109/10715769609088032

Abstract

Profiles of the local nitric oxide (.NO) diffusion-concentration product across the egg yolk phosphatidylcholine membrane in the absence and presence of 30 mol% cholesterol were obtained using line-broadening electron paramagnetic resonance (EPR) and lipid-soluble nitroxide spin labels. Membrane .NO permeability coefficients were calculated from these profiles. At 20 degrees C, values of 93 and 77 cm/s for membranes in the absence and presence of cholesterol were obtained, compared with 73 and 66 cm/s for water layers of the same thickness as the membranes. Fluid-phase membranes are not barriers to .NO transport. Cholesterol significantly increases .NO transport in the center of the lipid bilayer.

Author List

Subczynski WK, Lomnicka M, Hyde JS

Author

Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Biological Transport
Diffusion
Electron Spin Resonance Spectroscopy
Lipid Bilayers
Molecular Probes
Nitric Oxide
Permeability
Phosphatidylcholines