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Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike protein. STAR Protoc 2020 12 18;1(3):100214

Date

12/31/2020

Pubmed ID

33377107

Pubmed Central ID

PMC7757665

DOI

10.1016/j.xpro.2020.100214

Scopus ID

2-s2.0-85104071101   5 Citations

Abstract

This protocol describes an integrated approach for analyzing site-specific N- and O-linked glycosylation of SARS-CoV-2 spike protein by mass spectrometry. Glycoproteomics analyzes intact glycopeptides to examine site-specific microheterogeneity of glycoproteins. Glycomics provides structural characterization on any glycan assignments by glycoproteomics. This procedure can be modified and applied to a variety of N- and/or O-linked glycoproteins. Combined with bioinformatics, the glycomics-informed glycoproteomics may be useful in generating 3D molecular dynamics simulations of certain glycoproteins alone or interacting with one another. For complete details on the use and execution of this protocol, please refer to Zhao etA al. (2020).

Author List

Rosenbalm KE, Tiemeyer M, Wells L, Aoki K, Zhao P

Author

Kazuhiro Aoki PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Glycomics
Glycoproteins
Glycosylation
Humans
Proteomics
Spike Glycoprotein, Coronavirus