Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I. Angew Chem Int Ed Engl 2013 Dec 02;52(49):13012-5

Date

10/17/2013

Pubmed ID

24129959

Pubmed Central ID

PMC3869382

DOI

10.1002/anie.201307095

Scopus ID

2-s2.0-84888597910   55 Citations

Abstract

A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gala??I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.

Author List

Mbua NE, Li X, Flanagan-Steet HR, Meng L, Aoki K, Moremen KW, Wolfert MA, Steet R, Boons GJ

Author

Kazuhiro Aoki PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Click Chemistry
Fibroblasts
Humans
N-Acetylneuraminic Acid
Polysaccharides
Recombinant Proteins
Sialic Acids
Sialyltransferases