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Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation. Ocul Immunol Inflamm 2016 Oct;24(5):579-93

Date

10/17/2015

Pubmed ID

26471144

Pubmed Central ID

PMC5268843

DOI

10.3109/09273948.2015.1056534

Scopus ID

2-s2.0-84945232664   9 Citations

Abstract

PURPOSE: To investigate the effect of the Rho-kinase inhibitor, Y27632, on pig corneal endothelial cell (pCEC) culture, and on inflammation and immune regulation of the responses of human cells to pCECs.

METHODS: pCECs were cultured with/without Y27632 to assess cell proliferation and in vitro wound healing assay. The level of MCP-1 and VEGF in pCECs stimulated with human TNF-I? were measured. Proliferation of human PBMCs stimulated with pCECs, and cytokine production in human T cells, and monocyte migration after stimulation were investigated.

RESULTS: Y27632 promoted pCEC proliferation, prevented pCEC death, and enhanced in vitro wound healing. After stimulation, there were significantly lower levels of MCP-1 and VEGF measured in pCECs cultured with Y27632, and significantly reduced human PBMC proliferation, cytokine production, and monocyte migration.

CONCLUSIONS: The application of the Rho-kinase inhibitor will be beneficial when culturing pCECs, and may provide a novel therapy to reduce inflammation after corneal xenotransplantation.

Author List

Lee W, Miyagawa Y, Long C, Zhang M, Cooper DK, Hara H

Author

Whayoung Lee MD Assistant Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amides
Animals
Antibody Formation
Apoptosis
Cell Proliferation
Cells, Cultured
Cytokines
Endothelium, Corneal
Enzyme Inhibitors
Enzyme-Linked Immunosorbent Assay
Inflammation
Keratitis
Monocytes
Phosphorylation
Pyridines
Real-Time Polymerase Chain Reaction
Swine
Transcription Factor RelA
Vascular Endothelial Growth Factor A
Wound Healing
rho-Associated Kinases