Cepheid Xpert Xpress Flu/RSV evaluation performed by minimally trained non-laboratory operators in a CLIA-waived environment. Diagn Microbiol Infect Dis 2022 Oct;104(2):115764
Date
08/03/2022Pubmed ID
35917666Pubmed Central ID
PMC9271353DOI
10.1016/j.diagmicrobio.2022.115764Scopus ID
2-s2.0-85135545220 (requires institutional sign-in at Scopus site) 1 CitationAbstract
The COVID-19 pandemic highlighted the significance of readily available and easily performed viral testing for surveillance during future infectious pandemics. The objectives of this study were: to assess the performance of the Xpert Xpress Flu and/or RSV test, a multiplex PCR assay for detecting influenza A and B virus and respiratory syncytial virus nucleic acids in respiratory tract specimens, relative to the Quidel Lyra Influenza A+B assay and the Prodesse ProFlu+ assay, and the system's ease of use by minimally trained operators. Overall, the Xpert Xpress Flu/RSV test demonstrated a high positive and negative percent agreement with the comparator assays, and was easy to use and interpret results, based on the operators' feedback. We concluded that the Xpert Xpress Flu/RSV test is sensitive, specific, and easy to use for the diagnosis of influenza and RSV by minimally trained operators and can be a valuable tool in future infectious clusters or pandemics.
Author List
Shihabuddin BS, Faron ML, Relich RF, Van Heukelom P, Mayne D, Staat MA, Selvarangan R, Hueschen LA, Wolk DM, House S, Harnett G, McGann K, Steele MT, Romero JR, Arms J, Lander O, Loeffelholz M, Strouts F, Cohen DMESH terms used to index this publication - Major topics in bold
HumansInfluenza A virus
Influenza B virus
Influenza, Human
Molecular Diagnostic Techniques
Nasopharynx
Pandemics
Real-Time Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Respiratory Syncytial Virus, Human
Sensitivity and Specificity