Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study. FEBS Lett 1997 Feb 17;403(2):127-30
Date
02/17/1997Pubmed ID
9042951DOI
10.1016/s0014-5793(97)00036-7Scopus ID
2-s2.0-0242502758 (requires institutional sign-in at Scopus site) 193 CitationsAbstract
Lucigenin (LC2+) is frequently used as a superoxide probe. To detect superoxide, lucigenin must be reduced to the lucigenin cation radical (LC.+). We show, using the phosphorylated spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), that lucigenin stimulates NADPH-dependent superoxide production by endothelial nitric oxide synthase (eNOS). The formation of the DEPMPO-superoxide adduct is calcium/calmodulin independent. DEPMPO-superoxide adduct formation is inhibited by diphenyleneiodonium and is abolished by superoxide dismutase. It is likely that eNOS/NADPH can reduce lucigenin to LC.+ which reduces oxygen to superoxide. Consequently, lucigenin cannot be used to measure superoxide formation.
Author List
Vásquez-Vivar J, Hogg N, Pritchard KA Jr, Martasek P, Kalyanaraman BAuthors
Neil Hogg PhD Associate Dean, Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin
Jeannette M. Vasquez-Vivar PhD Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AcridinesAnimals
Cattle
Electron Spin Resonance Spectroscopy
Molecular Probes
NADP
Nitric Oxide Synthase
Oxidation-Reduction
Superoxides