Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning. J Biomol NMR 2020 May;74(4-5):267-285
Date
04/26/2020Pubmed ID
32333193Pubmed Central ID
PMC7236978DOI
10.1007/s10858-020-00316-yScopus ID
2-s2.0-85083825531 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of 1H and 13C nuclei. We also expand this approach to record 1H-, 13C-, and 15N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.
Author List
Gopinath T, Weber DK, Veglia GAuthor
Gopinath Tata PhD Assistant Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Carbon IsotopesHydrogen
Nuclear Magnetic Resonance, Biomolecular
Time Factors
