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Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences. J Am Chem Soc 2010 Apr 21;132(15):5357-63

Date

03/30/2010

Pubmed ID

20345172

Pubmed Central ID

PMC3328406

DOI

10.1021/ja905991s

Scopus ID

2-s2.0-77951040333 (requires institutional sign-in at Scopus site)   25 Citations

Abstract

We present new sensitivity enhanced schemes for heteronuclear correlation spectroscopy (HETCOR) in solid-state NMR of oriented systems. These schemes will enhance the sensitivity of the HETCOR by 40% for the two-dimensional experiments (SE-HETCOR) and up to 180% for the 3D HETCOR-separated local field version (SE-PISEMAI-HETCOR). The signal enhancement is demonstrated for a single crystal of ((15)N)N-acetylleucine and the integral membrane protein sarcolipin oriented in lipid bicelles. These methods will significantly reduce the time needed to acquire multidimensional experiments for membrane proteins oriented in magnetically or mechanically aligned lipid bilayers as well as liquid crystalline materials.

Author List

Gopinath T, Traaseth NJ, Mote K, Veglia G

Author

Gopinath Tata PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Leucine
Lipid Bilayers
Models, Chemical
Muscle Proteins
Nuclear Magnetic Resonance, Biomolecular
Proteolipids