Membrane orientation of the Na,K-ATPase regulatory membrane protein CHIF determined by solid-state NMR. Magn Reson Chem 2007 Dec;45 Suppl 1(Suppl 1):S192-7
Date
12/22/2007Pubmed ID
18098352Pubmed Central ID
PMC2920893DOI
10.1002/mrc.2144Scopus ID
2-s2.0-38849201806 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for (15)N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane.
Author List
Franzin CM, Teriete P, Marassi FMAuthor
Francesca M. Marassi PhD Chair, Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell MembraneHumans
Lipid Bilayers
Membrane Proteins
Micelles
Nuclear Magnetic Resonance, Biomolecular
Protein Conformation
