Faculty Collaboration Database

hnRNP C increases amyloid precursor protein (APP) production by stabilizing APP mRNA. Nucleic Acids Res 1998 Jul 15;26(14):3418-23

Date

07/03/1998

Pubmed ID

9649628

Pubmed Central ID

PMC147701

DOI

10.1093/nar/26.14.3418

Scopus ID

2-s2.0-0032528304 (requires institutional sign-in at Scopus site)   104 Citations

Abstract

We have previously shown that heterogeneous nuclear ribonucleoprotein C (hnRNP C) and nucleolin bound specifically to a 29 nt sequence in the 3'-untranslated region of amyloid precursor protein (APP) mRNA. Upon activation of peripheral blood mononuclear cells, hnRNP C and nucleolin acquired APP mRNA binding activity, concurrent with APP mRNA stabilization. These data suggested that the regulated interaction of hnRNP C and nucleolin with APP mRNA controlled its stability. Here we have directly examined the role of the cis element and trans factors in the turnover and translation of APP mRNA in vitro . In a rabbit reticulocyte lysate (RRL) translation system, a mutant APP mRNA lacking the 29 nt element was 3-4-fold more stable and synthesized 2-4-fold more APP as wild-type APP mRNA. Therefore, the 29 nt element functioned as an APP mRNA destabilizer. RNA gel mobility shift assays with the RRL suggested the presence of endogenous nucleolin, but failed to show hnRNP C binding activity. However, wild-type APP mRNA was stabilized and coded for 6-fold more APP when translated in an RRL system supplemented with exogenous active hnRNP C. Control mRNAs lacking the 29 nt element were unaffected by hnRNP C supplementation. Therefore, occupancy of the 29 nt element by hnRNP C stabilized APP mRNA and enhanced its translation.

Author List

Rajagopalan LE, Westmark CJ, Jarzembowski JA, Malter JS

Author

Jason A. Jarzembowski MD, PhD Sr Associate Dean, CEO CSG, Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amyloid beta-Protein Precursor
Base Sequence
Cell-Free System
Heterogeneous-Nuclear Ribonucleoprotein Group C
Heterogeneous-Nuclear Ribonucleoproteins
Protein Biosynthesis
RNA, Messenger
Ribonucleoproteins