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Limited variability of glycoprotein gene sequences and neutralizing targets in herpes simplex virus type 2 isolates and stability on passage in cell culture. J Infect Dis 1998 Jul;178(1):8-15

Date

07/04/1998

Pubmed ID

9652417

DOI

10.1086/515590

Scopus ID

2-s2.0-0031868159 (requires institutional sign-in at Scopus site)   37 Citations

Abstract

Nucleotide sequence analyses of polymerase chain reaction-amplified genes were performed to determine whether adaptation of herpes simplex virus type 2 to replication in cultured cells or in internal organs during neonatal disseminated disease results in selection of variants with altered forms of three glycoproteins (gB, gC, or gD) that influence virus entry into cells. No variations in sequence were noted as a consequence of in vitro passage or replication in different organs. Five viruses from different subjects differed with respect to gB, gC, and gD gene sequences, expressing four distinct forms of gB, three of gC, and two of gD. These differences did not confer resistance to neutralization by guinea pig or human antisera from subjects immunized with recombinant gB or gD vaccines and may not be consequential for vaccine development.

Author List

Terhune SS, Coleman KT, Sekulovich R, Burke RL, Spear PG

Author

Scott Terhune PhD Professor in the Microbiology and Immunology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adult
Animals
Antibodies, Viral
Cell Line
Cricetinae
Genes, Viral
Genetic Variation
Guinea Pigs
Herpes Genitalis
Herpesvirus 2, Human
Humans
Infant
Neutralization Tests
Sequence Analysis, DNA
Serial Passage
Tumor Cells, Cultured
Vaccines, Synthetic
Viral Envelope Proteins
Viral Vaccines
Virus Cultivation