The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state. Cell Rep 2022 Nov 01;41(5):111578
Date
11/03/2022Pubmed ID
36323247DOI
10.1016/j.celrep.2022.111578Scopus ID
2-s2.0-85140871739 (requires institutional sign-in at Scopus site) 13 CitationsAbstract
Long-term maintenance of the adult neurogenic niche depends on proper regulation of entry and exit from quiescence. Neural stem cell (NSC) transition from quiescence to activation is a complex process requiring precise cell-cycle control coordinated with transcriptional and morphological changes. How NSC fate transitions in coordination with the cell-cycle machinery remains poorly understood. Here we show that the Rb/E2F axis functions by linking the cell-cycle machinery to pivotal regulators of NSC fate. Deletion of Rb family proteins results in activation of NSCs, inducing a transcriptomic transition toward activation. Deletion of their target activator E2Fs1/3 results in intractable quiescence and cessation of neurogenesis. We show that the Rb/E2F axis mediates these fate transitions through regulation of factors essential for NSC function, including REST and ASCL1. Thus, the Rb/E2F axis is an important regulator of NSC fate, coordinating cell-cycle control with NSC activation and quiescence fate transitions.
Author List
Fong BC, Chakroun I, Iqbal MA, Paul S, Bastasic J, O'Neil D, Yakubovich E, Bejjani AT, Ahmadi N, Carter A, Clark A, Leone G, Park DS, Ghanem N, Vandenbosch R, Slack RSAuthor
Gustavo Leone PhD Sr Associate Dean, Director, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adult Stem CellsCell Cycle
Cell Division
Neural Stem Cells
Neurogenesis
Retinoblastoma Protein
