LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells. Nat Commun 2013;4:1531
Date
02/28/2013Pubmed ID
23443559DOI
10.1038/ncomms2517Scopus ID
2-s2.0-84874589546 (requires institutional sign-in at Scopus site) 62 CitationsAbstract
Centrosome morphology and number are frequently deregulated in cancer cells. Here, to identify factors that are functionally relevant for centrosome abnormalities in cancer cells, we established a protein-interaction network around 23 centrosomal and cell-cycle regulatory proteins, selecting the interacting proteins that are deregulated in cancer for further studies. One of these components, LGALS3BP, is a centriole- and basal body-associated protein with a dual role, triggering centrosome hypertrophy when overexpressed and causing accumulation of centriolar substructures when downregulated. The cancer cell line SK-BR-3 that overexpresses LGALS3BP exhibits hypertrophic centrosomes, whereas in seminoma tissues with low expression of LGALS3BP, supernumerary centriole-like structures are present. Centrosome hypertrophy is reversed by depleting LGALS3BP in cells endogenously overexpressing this protein, supporting a direct role in centrosome aberration. We propose that LGALS3BP suppresses assembly of centriolar substructures, and when depleted, causes accumulation of centriolar complexes comprising CPAP, acetylated tubulin and centrin.
Author List
Fogeron ML, Müller H, Schade S, Dreher F, Lehmann V, Kühnel A, Scholz AK, Kashofer K, Zerck A, Fauler B, Lurz R, Herwig R, Zatloukal K, Lehrach H, Gobom J, Nordhoff E, Lange BMAuthor
Alexandra K. Scholz PsyD Assistant Professor in the Psychiatry and Behavioral Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Neoplasm
Biomarkers, Tumor
Carrier Proteins
Cell Line, Tumor
Centrioles
Chromatography, Affinity
Extracellular Matrix Proteins
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Glycoproteins
HEK293 Cells
Humans
Hypertrophy
Male
Microtubules
Neoplasms
Protein Interaction Maps
Protein Transport
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Seminoma
Spindle Apparatus
