Autologous CD34+ stem cell humanization in head and neck cancer modeling: The translational importance of T cell maturation Oral Oncology Reports 2022, 100010. ISSN 2772-9060, https://doi.org/10.1016/j.oor.2022.100010.
Date
12/30/2022Abstract
Head and neck cancers (HNC) are aggressive malignancies with a poor prognosis. Immuno-oncologic agents have shown promise in HNC, but response rates remain low and predictive biomarkers are lacking [1,2]. To identify resistance mechanisms and develop precision immunotherapies, in vivo models that recapitulate the tumor-immune microenvironment are needed. To achieve this, patient-derived xenograft (PDX) models can be given a human immune system (humanized) through injection of CD34+ hematopoietic stem cells (HSCs). These HSCs, however, are typically obtained from separate donors, leading to HLA-mismatch between the PDX tumor and engrafted immune system. To overcome this challenge, we used patient-specific CD34+ HSCs from fibula bone marrow obtained during HNC surgery.
Author List
Zenga J, Awan MJ, Frei A, Shukla M, Shreenivas A, Wong SJ, Himburg HAAuthor
Heather A. Himburg PhD Associate Professor in the Radiation Oncology department at Medical College of WisconsinView Online