Intraperitoneal injection of MSC-derived exosomes prevent experimental bronchopulmonary dysplasia. Biochem Biophys Res Commun 2018 Sep 18;503(4):2653-2658
Date
08/11/2018Pubmed ID
30093115Pubmed Central ID
PMC6398932DOI
10.1016/j.bbrc.2018.08.019Scopus ID
2-s2.0-85051014878 (requires institutional sign-in at Scopus site) 87 CitationsAbstract
Mesenchymal stromal cell (MSC) derived exosomes mediate tissue protection and regeneration in many injuries and diseases by modulating cell protein production, protecting from apoptosis, inhibiting inflammation, and increasing angiogenesis. In the present study, daily intraperitoneal injection of MSC-derived exosomes protected alveolarization and angiogenesis in a newborn rat model of bronchopulmonary dysplasia (BPD) induced by 14 days of neonatal hyperoxia exposure (85% O2). Exosome treatment during hyperoxia prevented disruption of alveolar growth, increased small blood vessel number, and inhibited right heart hypertrophy at P14, P21, and P56. In vitro, exosomes significantly increased tube-like network formation by HUVEC, in part through a VEGF mediated mechanism. In summary, daily intraperitoneal injection of exosomes increased blood vessel number and size in the lung through pro-angiogenic mechanisms. MSC-derived exosomes therefore have both anti-inflammatory and pro-angiogenic mechanism to protect the lung from hyperoxia induced lung and heart disease associated with BPD.
Author List
Braun RK, Chetty C, Balasubramaniam V, Centanni R, Haraldsdottir K, Hematti P, Eldridge MWAuthor
Peiman Hematti MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Newborn
Bone Marrow Cells
Bronchopulmonary Dysplasia
Cardiomegaly
Disease Models, Animal
Exosomes
Female
Gene Expression Regulation
Hyperoxia
Injections, Intraperitoneal
Lung
Neovascularization, Physiologic
Oxygen
Pregnancy
Primary Cell Culture
Rats
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A