Release of CuPTSM from human serum albumin after addition of fatty acids. J Inorg Biochem 1996 Mar;61(4):251-9
Date
03/01/1996Pubmed ID
8867452DOI
10.1016/0162-0134(95)00072-0Scopus ID
2-s2.0-0030000738 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
Copper-62 labeled pyruvaldehyde bis (N4-methyl-thiosemicarbazonato) copper(II), CuPTSM, has been used to probe tissue perfusion by means of positron emission tomography. Despite promising results from animals, problems have been encountered in the use of 62CuPTSM to quantitate regional myocardial blood flow in humans. Ultrafiltration and plasma/erythrocyte partitioning studies with radiotracer have previously shown that CuPTSM is bound much more strongly by human serum albumin (HSA) than by dog serum albumin (DSA), limiting its ability to freely diffuse from blood into tissue. In this study, it is confirmed by electron spin resonance (ESR) that CuPTSM strongly binds to HSA with an apparent gparallel value of 2.12 and an apparent Aparallel value of 186 G. It is also shown that both spin-labeled stearic acid (5-SASL) and nonspin-labeled stearate inhibit CuPTSM binding to HSA. CuPTSM is completely released from HSA when the ratio of 5-SASL to HSA is 5:1. When pure sodium stearate is used, the binding of CuPTSM significantly decreased, about 73% of CuPTSM is released with a ratio of 4:1 stearate to HSA. These results highlight a means of liberating CuPTSM from HSA.
Author List
Yuan H, Antholine WE, Subczynski WK, Green MAAuthor
Witold K. Subczynski PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Electron Spin Resonance SpectroscopyFatty Acids
Humans
Organometallic Compounds
Protein Binding
Serum Albumin
Thiosemicarbazones