Medical College of Wisconsin
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Characterization of the human platelet N- and O-glycome upon storage using tandem mass spectrometry. Blood Adv 2023 Aug 22;7(16):4278-4290

Date

03/24/2023

Pubmed ID

36952551

Pubmed Central ID

PMC10424148

DOI

10.1182/bloodadvances.2022007084

Scopus ID

2-s2.0-85161978571 (requires institutional sign-in at Scopus site)   14 Citations

Abstract

Changes in surface glycan determinants, specifically sialic acid loss, determine platelet life span. The gradual loss of stored platelet quality is a complex process that fundamentally involves carbohydrate structures. Here, we applied lipophilic extraction and glycan release protocols to sequentially profile N- and O-linked glycans in freshly isolated and 7-day room temperature-stored platelet concentrates. Analytical methods including matrix assisted laser desorption/ionization time-of-flight mass spectrometry, tandem mass spectrometry, and liquid chromatography were used to obtain structural details of selected glycans and terminal epitopes. The fresh platelet repertoire of surface structures revealed diverse N-glycans, including high mannose structures, complex glycans with polylactosamine repeats, and glycans presenting blood group epitopes. The O-glycan repertoire largely comprised sialylated and fucosylated core-1 and core-2 structures. For both N- and O-linked glycans, we observed a loss in sialylated epitopes with a reciprocal increase in neutral structures as well as increased neuraminidase activity after platelet storage at room temperature. The data indicate that loss of sialylated glycans is associated with diminished platelet quality and untimely removal of platelets after storage.

Author List

Rosenbalm KE, Lee-Sundlov MM, Ashline DJ, Grozovsky R, Aoki K, Hanneman AJS, Hoffmeister KM

Author

Kazuhiro Aoki PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Blood Platelets
Epitopes
Humans
Polysaccharides
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry