Faculty Collaboration Database

Preparation of Der p 1 specific monoclonal antibodies and use in a two-site-ELISA to detect Der p 1 allergen. J Microbiol Immunol Infect 2000 Jun;33(2):87-92

Date

08/05/2000

Pubmed ID

10917877

Scopus ID

2-s2.0-0033942242 (requires institutional sign-in at Scopus site)   3 Citations

Abstract

It has been well documented that the mite Dermatophoides pteronyssinus (Der p) is the major allergen in house dust in Taiwan. The purpose of this study is to develop a sandwich immunometric assay which can be used for the standardization of mite allergen extracts or house dust, for assessing the procedures of mite avoidance, and for identifying species in epidemiological studies. Der p 1 allergen (both recombinant and native Der p 1) was purified and a panel of Der p 1 specific monoclonal antibodies was developed. The monoclonal antibodies were biotinylated and a two-site enzyme-linked immunosorbent assay (ELISA) with peroxidase-conjugated streptavidin was developed for the detection of Der p 1 allergen. The results suggest that these monoclonal antibodies could be applied both in affinity columns for the purification of native Der p 1 antigen and in sandwich-ELISA for epidemiological study.

Author List

Chien TI, Chen YG, Chiang BL

Author

Yi-Guang Chen PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Allergens
Animals
Antibodies, Monoclonal
Antigens, Dermatophagoides
Enzyme-Linked Immunosorbent Assay
Glycoproteins
Mice
Mice, Inbred BALB C
Mites
Recombinant Proteins