Medical College of Wisconsin
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Characterization of monoclonal antibodies against hepatitis C virus nonstructural protein 3: different antigenic determinants from human B cells. J Med Virol 1999 Apr;57(4):345-50

Date

03/24/1999

Pubmed ID

10089044

DOI

10.1002/(sici)1096-9071(199904)57:4<345::aid-jmv3>3.0.co;2-n

Scopus ID

2-s2.0-84984567059   7 Citations

Abstract

The nonstructural (NS3) region protein of hepatitis C virus (HCV) possesses major B-cell epitopes that induce antibodies after infection. To elucidate further the characteristics of these B cells and their role in the immune regulation of HCV infection, T9 (portion of NS3 region, amino acids [a.a.] 1188-1493)-specific monoclonal antibodies were derived and mapped for B-cell antigenic determinants with recombinant proteins. A total of 10 T9-specific hybridomas were generated and tested for B-cell antigenic determinants. To analyze the B-cell antigenic determinants, eight recombinant proteins including NS3-e (a.a. 1175-1334), NS3-a' (a.a. 1175-1250), NS3-a (a.a. 1251-1334), NS3-b (a.a. 1323-1412), NS3-c (a.a. 1407-1499), NS3-a/b (a.a. 1251-1412), NS3-bc (a.a. 1323-1499), and NS3-abc (a.a. 1251-1499) encoded by NS3-region internal clones were expressed and tested for immunoblotting. The data suggested IgG hybridomas recognized NS3-a, NS3-a', or NS3-b protein by immunoblotting. By contrast, the NS3-e protein bears the major antigenic determinant recognized by human sera. Half of the hybridomas were found to react with protein NS3-a', which is not a major B-cell antigenic determinant in humans. These data suggested that conformational epitopes in vivo may be important for B-cell recognition.

Author List

Ou-Yang P, Chiang BL, Hwang LH, Chen YG, Yang PM, Chi WK, Chen PJ, Chen DS

Author

Yi-Guang Chen PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Antibodies, Viral
Antibody Specificity
B-Lymphocytes
Enzyme-Linked Immunosorbent Assay
Epitopes, B-Lymphocyte
Fluorescent Antibody Technique
Hepacivirus
Humans
Immunoblotting
Mice
Mice, Inbred BALB C
Recombinant Proteins
Viral Nonstructural Proteins