Faculty Collaboration Database

Experimental spinal fusion with recombinant human bone morphogenetic protein-2 without decortication of osseous elements. Spine (Phila Pa 1976) 1997 Jun 01;22(11):1171-80

Date

06/01/1997

Pubmed ID

9201852

DOI

10.1097/00007632-199706010-00001

Scopus ID

2-s2.0-0030992841 (requires institutional sign-in at Scopus site)   110 Citations

Abstract

STUDY DESIGN: L4-L5 intertransverse process fusions were produced with 58 micrograms, 230 micrograms, or 920 micrograms of recombinant human bone morphogenetic protein-2 in 20 dogs. Eleven had traditional decortication of posterior elements before insertion of the implant. Nine were left undecorticated. All animals were evaluated 3 months after surgery.

OBJECTIVES: To determine whether decortication is a prerequisite for successful fusion in the presence of osteoinductive proteins such as bone morphogenetic protein-2.

SUMMARY OF BACKGROUND DATA: Recombinant osteoinductive proteins can induce de novo bone in ectopic soft-tissue sites in the absence of bone marrow elements. Traditional methods for achieving spinal fusion rely on exposure of bone marrow through decortication to facilitate osteogenesis. It is hypothesized that the presence of an implanted osteoinductive protein obviates the need for exposure and release of host inductive factors.

METHODS: Recombinant human bone morphogenetic protein-2-induced intertransverse process fusions were performed with and without decortication. Fusion sites were evaluated by computed tomography imaging, high-resolution radiography, manual testing, mechanical testing, and histologic analysis.

RESULTS: One hundred percent of decorticated spines and 89% of undecorticated spines were clinically fused by 3 months. Ninety-one percent of decorticated spines and 78% of undecorticated specimens exhibited bilateral transverse process osseous bridging. The only spines that failed to achieve solid bilateral arthrodesis were in the lowest dose group. With the higher two doses, there was histologic evidence of osseous continuity between the fusion mass and undecorticated transverse processes.

CONCLUSIONS: There were no statistical differences in clinical and radiographic fusion rates between decorticated and undecorticated sites. With higher doses of recombinant human bone morphoganetic protein-2, there was little histologic distinction between fusions in decorticated versus undecorticated spines.

Author List

Sandhu HS, Kanim LE, Toth JM, Kabo JM, Liu D, Delamarter RB, Dawson EG

Author

Jeffrey M. Toth PhD Associate Dean for Research in the School of Dentistry department at Marquette University

MESH terms used to index this publication - Major topics in bold

Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins
Bone Regeneration
Dogs
Female
Humans
Lumbar Vertebrae
Radiography
Recombinant Proteins
Spinal Fusion
Stress, Mechanical
Transforming Growth Factor beta