Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond. Development 2018 Mar 08;145(5)
Date
03/10/2018Pubmed ID
29519889Pubmed Central ID
PMC5868991DOI
10.1242/dev.156166Scopus ID
2-s2.0-85044319384 (requires institutional sign-in at Scopus site) 234 CitationsAbstract
The advent of human induced pluripotent stem cells (iPSCs) presents unprecedented opportunities to model human diseases. Differentiated cells derived from iPSCs in two-dimensional (2D) monolayers have proven to be a relatively simple tool for exploring disease pathogenesis and underlying mechanisms. In this Spotlight article, we discuss the progress and limitations of the current 2D iPSC disease-modeling platform, as well as recent advancements in the development of human iPSC models that mimic in vivo tissues and organs at the three-dimensional (3D) level. Recent bioengineering approaches have begun to combine different 3D organoid types into a single '4D multi-organ system'. We summarize the advantages of this approach and speculate on the future role of 4D multi-organ systems in human disease modeling.
Author List
Liu C, Oikonomopoulos A, Sayed N, Wu JCAuthor
Chun Liu PhD Assistant Professor in the Physiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
BioengineeringBiomimetic Materials
Cell Culture Techniques
Cell Differentiation
Disease
Extracellular Matrix
Humans
Induced Pluripotent Stem Cells
Models, Theoretical
Organoids
Tissue Scaffolds
