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Surface biofilm disruption. Enhanced recovery of microorganisms from vascular prostheses. Arch Surg 1987 Jan;122(1):38-43



Pubmed ID




Scopus ID

2-s2.0-0023089833   74 Citations


Ultrasonic oscillation (sonication) of explanted vascular prosthetic graft material can disrupt surface biofilms and increase the recovery of adherent microorganisms. Recovery of microorganisms from vascular grafts was studied in a canine model of Staphylococcus epidermidis graft contamination (N = 26) and on graft material excised from patients undergoing femoral anastomotic pseudoaneurysm repair (N = 7). Surface biofilm disruption by sonication significantly increased the incidence of positive cultures of excised graft material compared with broth (P less than .010) and blood agar plate (P less than .005) culture techniques. The S epidermidis was recovered from 31% of the canine vascular grafts and 100% of the clinical specimens. The in vitro production of a glycocalyx "slime" was demonstrated in 73% of the recovered staphylococcal strains. The formation of an adherent bacteria biofilm on implanted vascular prostheses is not an uncommon occurrence and is an important factor in the pathogenesis of anastomotic pseudoaneurysm formation and late graft infection.

Author List

Tollefson DF, Bandyk DF, Kaebnick HW, Seabrook GR, Towne JB


Gary R. Seabrook MD Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Bacteriological Techniques
Blood Vessel Prosthesis
Coronary Artery Bypass
Disease Models, Animal
Equipment Contamination
Microscopy, Electron, Scanning
Polysaccharides, Bacterial
Staphylococcal Infections
Staphylococcus epidermidis
jenkins-FCD Prod-480 9a4deaf152b0b06dd18151814fff2e18f6c05280