Faculty Collaboration Database

Critical role of ESCRT machinery in EGFR recycling. Biochemistry 2009 Oct 13;48(40):9321-3

Date

08/14/2009

Pubmed ID

19673488

DOI

10.1021/bi900865u

Scopus ID

2-s2.0-70350056841 (requires institutional sign-in at Scopus site)   45 Citations

Abstract

The molecular mechanisms of EGFR vesicular trafficking to lysosomes have recently received considerable attention. It is now clear that endosomal sorting complexes required for transport (ESCRTs) are critical for EGFR degradation. Although an increasing number of membrane receptors also undergo recycling via specific pathways, little information is available regarding regulated recycling of EGFR. In this study, we investigated the roles of ESCRTs in EGFR recycling after stimulation with amphiregulin (AR). We used ESCRT small interfering RNA (siRNA) duplexes to demonstrate that AR-induced EGFR intracellular processing involves active sorting to the recycling pathway through specific members of the ESCRT family.

Author List

Baldys A, Raymond JR

Author

John R. Raymond MD President, CEO, Professor in the President department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amphiregulin
DNA-Binding Proteins
EGF Family of Proteins
Endocytosis
Endosomal Sorting Complexes Required for Transport
Endosomes
ErbB Receptors
Glycoproteins
Humans
Intercellular Signaling Peptides and Proteins
Ligands
Membrane Proteins
Multiprotein Complexes
Phosphoproteins
Protein Transport
Proto-Oncogene Proteins c-cbl
Receptor Protein-Tyrosine Kinases
Transcription Factors
Ubiquitin
Vesicular Transport Proteins