An improved growth hormone reporter gene shows a different pattern of transactivation by promoter fragments. Gene 2003 Dec 24;323:173-80
Date
12/09/2003Pubmed ID
14659891DOI
10.1016/j.gene.2003.09.020Scopus ID
2-s2.0-0344628549 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Granulocytic differentiation of myeloid NB4 cells was noted to transactivate the genomic human growth hormone (hGH) reporter construct in the absence of a driving test promoter. Such spurious hGH transactivation also occurred upon co-transfection of plasmids expressing the transactivators c-Myb and C/EBPalpha into undifferentiated NB4 cells. Modifications to the reporter gene and the vector resulted in a reporter construct unaffected by such stimuli. When human neutrophil peptide 1 (hnp-1) gene promoter fragments were tested using both the original and modified reporter constructs, a clear difference in the pattern of transactivation was noted. This suggests that promoter and enhancer elements in the reporter construct plasmid DNA can effect the analysis of the test promoter.
Author List
Schembri-Wismayer P, Birnie GDAuthor
Jordan Gliedt DC Associate Professor in the Neurosurgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Cell Line, TumorGene Expression
Genes, Reporter
Genetic Vectors
HL-60 Cells
Human Growth Hormone
Humans
Mutation
Promoter Regions, Genetic
Recombinant Fusion Proteins
Sequence Deletion
Transcriptional Activation
Transfection
Up-Regulation
