Liposome-mediated transfection with extract from neonatal rat cardiomyocytes induces transdifferentiation of human adipose-derived stem cells into cardiomyocytes. Scand J Clin Lab Invest 2008;68(6):464-72
Date
07/09/2008Pubmed ID
18609095DOI
10.1080/00365510701836907Scopus ID
2-s2.0-52949100519 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
OBJECTIVE: Recent studies indicate that direct cell-to-cell interaction is involved in transdifferentiation of adult stem cells into cardiomyocytes. We investigated whether transdifferentiation of human adipose-tissue-derived stem cells could be achieved by transfecting the cells with a nuclear neonatal cardiomyocyte extract using a liposome-based transfection system.
MATERIAL AND METHODS: In this study, we isolated stem cells derived from human subcutaneous adipose tissue. These cells were transfected with nuclear protein extracts from either isolated cardiomyocytes or whole hearts of neonatal rats. Results. We found that transfection induced expression of the cardiac markers alpha-sarcomeric actin, Nkx2.5, troponin I and troponin T after 1-3 weeks. Whole-heart protein extracts showed the additional capacity to induce differentiation into endothelial-like and smooth muscle-like cells.
CONCLUSION: We demonstrate that transfection with nuclear protein extracts from neonatal rat cardiomyocytes can induce a cardiomyogenic differentiation pathway in human stem cells.
Author List
Schimrosczyk K, Song YH, Vykoukal J, Vykoukal D, Bai X, Coleman M, Krohn A, Freyberg S, Alt EUAuthor
Xiaowen Bai PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdipocytesAnimals
Animals, Newborn
Cell Differentiation
Cell Lineage
Cell Transdifferentiation
Cells, Cultured
Endothelial Cells
Gene Expression Regulation
Humans
Liposomes
Myocytes, Cardiac
Proteins
RNA, Messenger
Rats
Stem Cells
Transfection
Transgenes