Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Protocol for the isolation of tumor cell-derived extracellular vesicles followed by in vivo metastasis assessment in a murine ovarian cancer model. STAR Protoc 2024 Jun 21;5(2):102943

Date

03/12/2024

Pubmed ID

38470912

Pubmed Central ID

PMC10945248

DOI

10.1016/j.xpro.2024.102943

Scopus ID

2-s2.0-85187377383 (requires institutional sign-in at Scopus site)

Abstract

Extracellular vesicles (EVs) play a crucial role in facilitating communication between cancer cells and their immediate or remote microenvironments, thereby promoting the extensive spread of cancer throughout the body. In this context, we present a protocol for the isolation of tumor cell-derived EVs followed by in vivo metastasis assessment in a murine ovarian cancer model. We describe steps for the isolation and characterization of EVs from ID8 cells, development of a metastatic mouse model, and sample preparation for flow cytometry. For complete details on the use and execution of this protocol, please refer to Gupta et al.1.

Author List

Mittal S, Kumar S, Gupta P, Singh M, Chaluvally-Raghavan P, Pradeep S

Author

Pradeep Chaluvally-Raghavan PhD Associate Professor in the Obstetrics and Gynecology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cell Line, Tumor
Disease Models, Animal
Extracellular Vesicles
Female
Flow Cytometry
Mice
Neoplasm Metastasis
Ovarian Neoplasms
Tumor Microenvironment