Faculty Collaboration Database

Attenuation of cardiac ischemia-reperfusion injury by sodium thiosulfate is partially dependent on the effect of cystathione beta synthase in the myocardium. Cell Biochem Biophys 2019 Sep;77(3):261-272

Date

05/09/2019

Pubmed ID

31065867

DOI

10.1007/s12013-019-00871-8

Scopus ID

2-s2.0-85065534360 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

Our early studies have shown that sodium thiosulfate (STS) treatment attenuated the ischemia-reperfusion (IR)-induced injury in an isolated rat heart model by decreasing apoptosis, oxidative stress, and preserving mitochondrial function. Hydrogen sulfide, the precursor molecule is reported to have similar efficacy. This study aims to investigate the role of endogenous hydrogen sulfide in STS-mediated cardioprotection against IR in an isolated rat heart model. D, L-propargylglycine (PAG), an inhibitor of cystathionine γ-lyase was used as endogenous H₂S blocker. In addition, we used the hypoxia-reoxygenation (HR) model to study the impact of STS in cardiomyocytes (H9C2) and fibroblast (3T3) cells. STS treatment to animal and cells prior to IR or HR decreased cell injury. The sensitivity of H9C2 and 3T3 cells towards HR (6 h hypoxia followed by 12 h reoxygenation) challenge varies, where, 3T3 cells exhibited higher cell death (54%). Cells treated with PAG prior to STS abrogate the protective effect in 3T3 (cell viability 61%) but not in H9C2 (cell viability 82%). Further evaluation in rat heart model showed partial recovery (46% RPP) of heart from those hearts pretreated with PAG prior to STS condition. In conclusion, we demonstrated that STS-mediated cardioprotection to IR-challenged rat heart is not fully dependent on endogenous H₂S level and this dependency may be linked to higher fibroblast content in rat heart.

Author List

Kannan S, Boovarahan SR, Rengaraju J, Prem P, Kurian GA

Author

Sri Rahavi Boovarahan PhD Postdoctoral Researcher 1 in the Biomedical Engineering department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alkynes
Animals
Antidotes
Antioxidants
Apoptosis
Cell Hypoxia
Cell Line
Cell Survival
Cystathionine gamma-Lyase
Glycine
Heart
Hydrogen Sulfide
Lipid Peroxidation
Male
Mice
Mitochondria
Myocardial Reperfusion Injury
Myocardium
Rats
Rats, Wistar
Thiosulfates