Utilization of HPASubC for the Identification of Sinusoid-Specific Proteins in the Liver. J Proteome Res 2016 May 06;15(5):1623-9
Date
03/24/2016Pubmed ID
27005832Pubmed Central ID
PMC8201909DOI
10.1021/acs.jproteome.6b00073Scopus ID
2-s2.0-84969242476 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Mass spectrometry-based proteomes of human organs and tissues are powerful tools but fail to capture protein localization and expression at the cellular level. For example, the proteome signal in liver represents the combined protein expression across diverse cellular constituents that include hepatocytes, Kupffer cells, endothelial cells, and others. We utilized HPASubC and the Human Protein Atlas (HPA) to identify the sinusoidal component of protein liver expression to further subset and organize this homogeneous signal. We evaluated 51 109 liver images covering 13 197 proteins from the HPA and discovered 1054 proteins that were exclusive to sinusoidal cells. Sinusoidal staining patterns were identified in a Kupffer cell (n = 247), endothelial cell (n = 358), or lymphocyte (n = 86) specific pattern. Two-hundred and thirty-nine of these proteins were not present in the NextProt or Human Proteome Map liver data sets, potentially expanding our knowledge of the liver proteome. We additionally demonstrate unique endothelial cell expression patterns that distinguish between portal vein, hepatic artery, capillary sinusoids, and central vein regions. These findings significantly improve our understanding of the liver proteome with insight into the endothelial complexity across the hepatic vascular network.
Author List
Anene DF, Rosenberg AZ, Kleiner DE, Cornish TC, Halushka MKAuthor
Toby Charles Cornish MD, PhD Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CapillariesData Mining
Endothelium, Vascular
Humans
Kupffer Cells
Liver
Lymphocytes
Proteome
