Faculty Collaboration Database

Software-automated counting of Ki-67 proliferation index correlates with pathologic grade and disease progression of follicular lymphomas. Am J Clin Pathol 2013 Oct;140(4):579-87

Date

09/21/2013

Pubmed ID

24045557

Pubmed Central ID

PMC4010253

DOI

10.1309/AJCPTMA1F6LWYTQV

Scopus ID

2-s2.0-84884919123 (requires institutional sign-in at Scopus site)   19 Citations

Abstract

OBJECTIVES: To examine the accuracy of software-assisted measurement of the Ki-67 proliferation index (PI) and its correlation with the grade and clinical progression of follicular lymphoma (FL).

METHODS: High-power field equivalents were extracted from H&E- and Ki-67-immunostained slides of FL, and a nuclear quantitation algorithm was used to calculate a PI. Representative fields were manually counted for validation with close agreement.

RESULTS: The PI was significantly higher in World Health Organization grade 3 FL than grade 1 to 2 FL. Disease progression, as defined by subsequent treatment with radiation or cytotoxic chemotherapy, was also significantly associated with elevated PI but not pathologic grade.

CONCLUSIONS: These data show that software-automated quantitation of Ki-67 can provide both a useful adjunct to pathologic grade in FL and improved prognostic information for patients.

Author List

Samols MA, Smith NE, Gerber JM, Vuica-Ross M, Gocke CD, Burns KH, Borowitz MJ, Cornish TC, Duffield AS

Author

Toby Charles Cornish MD, PhD Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Aged, 80 and over
Automation, Laboratory
Cell Nucleus
Cell Proliferation
Disease Progression
Female
Humans
Ki-67 Antigen
Lymph Nodes
Lymphoma, Follicular
Male
Middle Aged
Neoplasm Grading
Prognosis
Reproducibility of Results
Software