Faculty Collaboration Database

Prenatal genotyping of the Duffy blood group system by allele-specific polymerase chain reaction. Prenat Diagn 1999 Jan;19(1):41-5

Date

03/12/1999

Pubmed ID

10073905

DOI

10.1002/(sici)1097-0223(199901)19:1<41::aid-pd463>3.0.co;2-a

Scopus ID

2-s2.0-0032935857 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

Maternal allo-immunization to antigens of the Duffy blood group system can result in haemolytic disease of the newborn (HDN), therefore, the application of allele-specific polymerase chain reaction (ASPCR) for prenatal genotyping of the Duffy antigen system to identify pregnancies at risk for HDN was evaluated. Oligonucleotide primers were designed for ASPCR of FYA, FYB and nullFY alleles. A validation study was performed using DNA isolated from 94 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the FYA, FYB and nullFY alleles. This assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to maternal fetal incompatibilities within the Duffy blood group system.

Author List

Hessner MJ, Pircon RA, Johnson ST, Luhm RA

Author

Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Alleles
Amniocentesis
Blood Group Incompatibility
DNA
Duffy Blood-Group System
Erythroblastosis, Fetal
Female
Genotype
Homozygote
Humans
Infant, Newborn
Polymerase Chain Reaction
Pregnancy
Risk Factors