Faculty Collaboration Database

Transcriptomic diversity of innate lymphoid cells in human lymph nodes compared to BM and spleen. Commun Biol 2024 Jun 25;7(1):769

Date

06/26/2024

Pubmed ID

38918571

Pubmed Central ID

PMC11199704

DOI

10.1038/s42003-024-06450-9

Scopus ID

2-s2.0-85196869670 (requires institutional sign-in at Scopus site)

Abstract

Innate lymphoid cells (ILCs) are largely tissue-resident, mostly described within the mucosal tissues. However, their presence and functions in the human draining lymph nodes (LNs) are unknown. Our study unravels the tissue-specific transcriptional profiles of 47,287 CD127⁺ ILCs within the human abdominal and thoracic LNs. LNs contain a higher frequency of CD127⁺ ILCs than in BM or spleen. We define independent stages of ILC development, including EILP and pILC in the BM. These progenitors exist in LNs in addition to naïve ILCs (nILCs) that can differentiate into mature ILCs. We define three ILC1 and four ILC3 sub-clusters in the LNs. ILC1 and ILC3 subsets have clusters with high heat shock protein-encoding genes. We identify previously unrecognized regulons, including the BACH2 family for ILC1 and the ATF family for ILC3. Our study is the comprehensive characterization of ILCs in LNs, providing an in-depth understanding of ILC-mediated immunity in humans.

Author List

Hashemi E, McCarthy C, Rao S, Malarkannan S

Authors

Subramaniam Malarkannan PhD Professor in the Medicine department at Medical College of Wisconsin
Sridhar Rao MD, PhD Associate Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Bone Marrow
Gene Expression Profiling
Humans
Immunity, Innate
Lymph Nodes
Lymphocytes
Male
Spleen
Transcriptome