Bare surface of gold nanoparticle induces inflammation through unfolding of plasma fibrinogen. Sci Rep 2018 Aug 22;8(1):12557
Date
08/24/2018Pubmed ID
30135553Pubmed Central ID
PMC6105630DOI
10.1038/s41598-018-30915-7Scopus ID
2-s2.0-85052533024 (requires institutional sign-in at Scopus site) 48 CitationsAbstract
The surface of nanoparticles (NPs) get coated by a wide range of biomolecules, upon exposure to biological fluids. It is now being increasingly accepted that NPs with particular physiochemical properties have a capacity to induce conformational changes to proteins and therefore influence their biological fates, we hypothesized that the gold NP's metal surface may also be involved in the observed Fg unfolding and inflammatory response. To mechanistically test this hypothesis, we probed the interaction of Fg with gold surfaces using molecular dynamic simulation (MD) and revealed that the gold surface has a capacity to induce Fg conformational changes in favor of inflammation response. As the integrity of coatings at the surface of ultra-small gold NPs are not thorough, we also hypothesized that the ultra-small gold NPs have a capacity to induce unfolding of Fg regardless of the composition and surface charge of their coatings. Using different surface coatings at the surface of ultra-small gold NPs, we validated this hypothesis. Our findings suggest that gold NPs may cause unforeseen inflammatory effects, as their surface coatings may be degraded by physiological activity.
Author List
Kharazian B, Lohse SE, Ghasemi F, Raoufi M, Saei AA, Hashemi F, Farvadi F, Alimohamadi R, Jalali SA, Shokrgozar MA, Hadipour NL, Ejtehadi MR, Mahmoudi MAuthor
Reza Alimohammadi Postdoctoral Researcher 2 in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
FibrinogenGold
Humans
Inflammation
Metal Nanoparticles
Molecular Dynamics Simulation
Protein Conformation
Protein Transport
Protein Unfolding
Surface Properties
