Faculty Collaboration Database

On the principle of ion selectivity in Na+/H+-coupled membrane proteins: experimental and theoretical studies of an ATP synthase rotor. Proc Natl Acad Sci U S A 2015 Mar 10;112(10):E1057-66

Date

02/26/2015

Pubmed ID

25713346

Pubmed Central ID

PMC4364180

DOI

10.1073/pnas.1421202112

Scopus ID

2-s2.0-84924401794 (requires institutional sign-in at Scopus site)   34 Citations

Abstract

Numerous membrane transporters and enzymes couple their mechanisms to the permeation of Na(+) or H(+), thereby harnessing the energy stored in the form of transmembrane electrochemical potential gradients to sustain their activities. The molecular and environmental factors that control and modulate the ion specificity of most of these systems are, however, poorly understood. Here, we use isothermal titration calorimetry to determine the Na(+)/H(+) selectivity of the ion-driven membrane rotor of an F-type ATP synthase. Consistent with earlier theoretical predictions, we find that this rotor is significantly H(+) selective, although not sufficiently to be functionally coupled to H(+), owing to the large excess of Na(+) in physiological settings. The functional Na(+) specificity of this ATP synthase thus results from two opposing factors, namely its inherent chemical selectivity and the relative availability of the coupling ion. Further theoretical studies of this membrane rotor, and of two others with a much stronger and a slightly weaker H(+) selectivity, indicate that, although the inherent selectivity of their ion-binding sites is largely set by the balance of polar and hydrophobic groups flanking a conserved carboxylic side chain, subtle variations in their structure and conformational dynamics, for a similar chemical makeup, can also have a significant contribution. We propose that the principle of ion selectivity outlined here may provide a rationale for the differentiation of Na(+)- and H(+)-coupled systems in other families of membrane transporters and enzymes.

Author List

Leone V, Pogoryelov D, Meier T, Faraldo-Gómez JD

Author

Vanessa Leone PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Binding Sites
Membrane Proteins
Molecular Sequence Data
Protein Binding
Protein Conformation
Proton-Translocating ATPases
Protons
Sequence Homology, Amino Acid
Sodium