Faculty Collaboration Database

Identification of a novel tumor suppressor gene p34 on human chromosome 6q25.1. Cancer Res 2007 Jan 01;67(1):93-9

Date

01/11/2007

Pubmed ID

17210687

Pubmed Central ID

PMC3461257

DOI

10.1158/0008-5472.CAN-06-2723

Scopus ID

2-s2.0-33846408685 (requires institutional sign-in at Scopus site)   36 Citations

Abstract

In this study, we observed loss of heterozygosity (LOH) in human chromosomal fragment 6q25.1 in sporadic lung cancer patients. LOH was observed in 65% of the 26 lung tumors examined and was narrowed down to a 2.2-Mb region. Single-nucleotide polymorphism (SNP) analysis of genes located within this region identified a candidate gene, termed p34. This gene, also designated as ZC3H12D, C6orf95, FLJ46041, or dJ281H8.1, carries an A/G nonsynonymous SNP at codon 106, which alters the amino acid from lysine to arginine. Nearly 73% of heterozygous lung cancer tissues with LOH and the A/G SNP also exhibited loss of the A allele. In vitro clonogenic and in vivo nude mouse studies showed that overexpression of the A allele exerts tumor suppressor function compared with the G allele. p34 is located within a recently mapped human lung cancer susceptibility locus, and association of the p34 A/G SNP was tested among these families. No significant association between the less frequent G allele and lung cancer susceptibility was found. Our results suggest that p34 may be a novel tumor suppressor gene involved in sporadic lung cancer but it seems not to be the candidate familial lung cancer susceptibility gene linked to chromosomal region 6q23-25.

Author List

Wang M, Vikis HG, Wang Y, Jia D, Wang D, Bierut LJ, Bailey-Wilson JE, Amos CI, Pinney SM, Petersen GM, de Andrade M, Yang P, Wiest JS, Fain PR, Schwartz AG, Gazdar A, Minna J, Gaba C, Rothschild H, Mandal D, Kupert E, Seminara D, Liu Y, Viswanathan A, Govindan R, Anderson MW, You M

MESH terms used to index this publication - Major topics in bold

Alleles
Animals
Base Sequence
Chromosomes, Human, Pair 6
Codon
Female
Genes, Tumor Suppressor
Genetic Predisposition to Disease
Humans
Loss of Heterozygosity
Lung Neoplasms
Mice
Mice, Nude
Molecular Sequence Data
Polymorphism, Single Nucleotide