Faculty Collaboration Database

An integrated method for direct interrogation of sphingolipid homeostasis in the heart and brain tissues of mice through postnatal development up to reproductive senescence. Anal Chim Acta 2018 Dec 11;1037:152-158

Date

10/08/2018

Pubmed ID

30292289

DOI

10.1016/j.aca.2018.01.015

Scopus ID

2-s2.0-85054183623 (requires institutional sign-in at Scopus site)   26 Citations

Abstract

Development of rapid metabolomic methods poised for pathway discovery is expected to facilitate the identification of therapeutic candidates in the metabolomic approach to translational medicine. Using sphingolipid homeostasis as a prototype, we present herein an integrated method to facilitate a fast interrogation of altered sphingolipid (and phospholipid) metabolism associated with perturbed endolysosomal functions in mammalian systems. Constructed upon high performance liquid chromatography coupled to mass spectrometry, this method allows semi-quantitative measurements of more than 300 individual species within 20 min. The method was applied to investigate cardiac- and neural-specific developmental changes in sphingolipid regulation from the postnatal stage to reproductive senescence in mice, revealing that endogenous lysobisphosphatidic acids and specific complex glycosphingolipids are tightly co-regulated to foster concerted reductions in sphingolipid levels at distinct stages of postnatal development. Our lipidomic data suggest that such changing regulatory patterns in sphingolipid homeostasis is attributed to differential endolysosomal degradation of complex sphingolipids, which may be critical in ensuring efficient sphingolipid catabolism and organismal health at each stage of postnatal development.

Author List

Lam SM, Wang R, Miao H, Li B, Shui G

Author

Huan Miao Postdoctoral Researcher 1 in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Brain
Chromatography, High Pressure Liquid
Fertility
Heart
Homeostasis
Mass Spectrometry
Metabolomics
Mice
Myocardium
Reproduction
Sphingolipids