A broadly applicable method for quantifying cardiomyocyte cell division identifies proliferative events following myocardial infarction. Cell Rep Methods 2024 Sep 16;4(9):100860
Date
09/11/2024Pubmed ID
39255794Pubmed Central ID
PMC11440799DOI
10.1016/j.crmeth.2024.100860Scopus ID
2-s2.0-85204511356 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Cardiomyocyte proliferation is a challenging metric to assess. Current methodologies have limitations in detecting the generation of new cardiomyocytes and technical challenges that reduce widespread applicability. Here, we describe an improved cell suspension and imaging-based methodology that can be broadly employed to assess cardiomyocyte cell division in standard laboratories across a multitude of model organisms and experimental conditions. We highlight additional metrics that can be gathered from the same cell preparations to enable additional relevant analyses to be performed. We incorporate additional antibody stains to address potential technical concerns of miscounting. Finally, we employ this methodology with a dual-thymidine analog-labeling approach to a post-infarction murine model, which allowed us to robustly identify unique cycling events, such as cardiomyocytes undergoing multiple rounds of cell division.
Author List
Swift SK, Purdy AL, Buddell T, Lovett JJ, Chanjeevaram SV, Arkatkar A, O'Meara CC, Patterson MAuthors
Caitlin C. O'Meara PhD Associate Professor in the Physiology department at Medical College of WisconsinMichaela Patterson PhD Associate Professor in the Cell Biology Neurobiology and Anatomy department at Medical College of Wisconsin
Alexandra L. Purdy Research Scientist I in the Cell Biology Neurobiology and Anatomy department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsCell Division
Cell Proliferation
Disease Models, Animal
Male
Mice
Mice, Inbred C57BL
Myocardial Infarction
Myocytes, Cardiac
