Faculty Collaboration Database

Expression of prox1, lymphatic endothelial nuclear transcription factor, in Kaposiform hemangioendothelioma and tufted angioma. Am J Surg Pathol 2010 Nov;34(11):1563-73

Date

10/27/2010

Pubmed ID

20975337

DOI

10.1097/PAS.0b013e3181f6076f

Scopus ID

2-s2.0-78049528230 (requires institutional sign-in at Scopus site)   84 Citations

Abstract

Kaposiform hemangioendothelioma (KHE) and tufted angioma (TA) are rare tumors mainly occurring in early childhood. Our recent results showed that ectopic overexpression of human Prox1 gene, a lymphatic endothelial nuclear transcription factor, promoted an aggressive behavior in 2 murine models of KHE. This dramatic Prox1-induced phenotype prompted us to investigate immunohistochemical staining pattern of Prox1, podoplanin (D2-40), LYVE-1, and Prox1/CD34 as well as double immunofluorescent staining pattern of LYVE-1/CD31 in KHE and TA, compared with other pediatric vascular tumors. For this purpose, we examined 75 vascular lesions: KHE (n=18), TA (n=13), infantile hemangioma (n=13), pyogenic granuloma (n=18), and granulation tissue (n=13). Overall, KHE and TA shared an identical endothelial immunophenotype: the neoplastic spindle cells were Prox1, podoplanin, LYVE-1, CD31, and CD34, whereas endothelial cells within glomeruloid foci were Prox1, podoplanin, LYVE-1, CD31, and CD34. The lesional cells of all infantile hemangiomas and pyogenic granulomas were negative for Prox1 in the presence of positive internal control. These findings provide immunophenotypic evidence to support a preexisting notion that KHE and TA are closely related, if not identical. Overall, our results show, for the first time, that Prox1 is an immunohistochemical biomarker helpful in confirming the diagnosis of KHE/TA and in distinguishing it from infantile hemangioma and pyogenic granuloma.

Author List

Le Huu AR, Jokinen CH, Rubin BP, Mihm MC, Weiss SW, North PE, Dadras SS

Author

Paula E. North MD, PhD Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adolescent
Antigens, CD34
Biomarkers, Tumor
Cell Nucleus
Child
Child, Preschool
Cytoplasm
Diagnosis, Differential
Granuloma, Pyogenic
Hemangioendothelioma
Hemangioma
Homeodomain Proteins
Humans
Immunohistochemistry
Immunophenotyping
Infant
Membrane Glycoproteins
Platelet Endothelial Cell Adhesion Molecule-1
Predictive Value of Tests
Skin Neoplasms
Soft Tissue Neoplasms
Tumor Suppressor Proteins
Vesicular Transport Proteins