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An early developmental transcription factor complex that is more stable on nucleosome core particles than on free DNA. Mol Cell 1999 Dec;4(6):961-9

Date

01/15/2000

Pubmed ID

10635321

DOI

10.1016/s1097-2765(00)80225-7

Scopus ID

2-s2.0-0033388099   177 Citations

Abstract

In vivo footprinting studies have shown that transcription factor binding sites for HNF3 and GATA-4 are occupied on the albumin gene enhancer in embryonic endoderm, prior to the developmental activation of liver gene transcription. We have investigated how these factors can stably occupy silent chromatin. Remarkably, we find that HNF3, but not GATA-4 or a GAL4 control protein, binds far more stably to nucleosome core particles than to free DNA. In the presence of HNF3, GATA-4 binds stably to an HNF3-positioned nucleosome. Histone acetylation does not affect HNF3 binding. This is evidence for stable nucleosome binding by a transcription factor and shows that a winged helix protein is sufficient to initiate the assembly of an enhancer complex on nonacetylated nucleosomes.

Author List

Cirillo LA, Zaret KS

Author

Lisa A. Cirillo PhD Assistant Dean, Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Binding Sites
Chromatin
DNA
DNA-Binding Proteins
Escherichia coli
Forkhead Transcription Factors
GATA4 Transcription Factor
Mice
Nucleosomes
Trans-Activators
Transcription Factors