Anti-inflammatory effects of freeze-dried black raspberry powder in ulcerative colitis. Carcinogenesis 2011 Mar;32(3):343-50
Date
11/26/2010Pubmed ID
21098643Pubmed Central ID
PMC3047236DOI
10.1093/carcin/bgq248Scopus ID
2-s2.0-79952285549 (requires institutional sign-in at Scopus site) 121 CitationsAbstract
Ulcerative colitis (UC) is a chronic inflammatory disease of the colonic mucosa that can dramatically increase the risk of colon cancers. In the present study, we evaluated the effects of a dietary intervention of freeze-dried black raspberries (BRB), a natural food product with antioxidant and anti-inflammatory bioactivities, on disease severity in an experimental mouse model of UC using 3% dextran sodium sulfate (DSS). C57BL/6J mice were fed either a control diet or a diet containing BRB (5 or 10%) for 7-14 days and then the extent of colonic injury was assessed. Dietary BRB markedly reduced DSS-induced acute injury to the colonic epithelium. This protection included better maintenance of body mass and reductions in colonic shortening and ulceration. BRB treatment, however, did not affect the levels of either plasma nitric oxide or colon malondialdehyde, biomarkers of oxidative stress that are otherwise increased by DSS-induced colonic injury. BRB treatment for up to 7 days suppressed tissue levels of several key pro-inflammatory cytokines, including tumor necrosis factor α and interleukin 1β. Further examination of the inflammatory response by western blot analysis revealed that 7 day BRB treatment reduced the levels of phospho-IκBα within the colonic tissue. Colonic cyclooxygenase 2 levels were also dramatically suppressed by BRB treatment, with a concomitant decrease in the plasma prostaglandin E₂ (276 versus 34 ng/ml). These findings demonstrate a potent anti-inflammatory effect of BRB during DSS-induced colonic injury, supporting its possible therapeutic or preventive role in the pathogenesis of UC and related neoplastic events.
Author List
Montrose DC, Horelik NA, Madigan JP, Stoner GD, Wang LS, Bruno RS, Park HJ, Giardina C, Rosenberg DWMESH terms used to index this publication - Major topics in bold
AnimalsAnti-Inflammatory Agents
Blotting, Western
Colitis, Ulcerative
Cytokines
Dextran Sulfate
Dinoprostone
Freeze Drying
Fruit
Immunoenzyme Techniques
Intestinal Mucosa
Male
Mice
Mice, Inbred C57BL
Oxidative Stress
Phosphorylation
Phytotherapy
Plant Extracts
Powders
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Rosaceae
